peptide mass fingerprinting meaning in Chinese
肽质指纹图谱
Examples
- Proteins were identified with peptide mass fingerprinting using matrix - assisted laser desorption ionization time of flight mass spectrometry ( maldi - tof ms ) after tryptic in - gel digestion
差异蛋白经胰蛋白酶酶切后产生肽片段,再利用基质辅助激光解吸电离飞行时间质谱得到肽指纹图谱来鉴定。 - Proteins were identified with peptide mass fingerprinting ( pmf ) . firstly , the first molecular anatomic profile of aeromonas hydrophila was established , 40 proteins were identitied from analysis of the pmf of l24 proteins . of the 40 proteins , 17 were from the source of aeromons hydrophila genes / proteins
实验首先初步构建了一张嗜水气单胞菌总蛋白质的分子解剖图谱,通过对124个蛋白质的肽质量指纹图谱分析,鉴定了40个蛋白质,其中17个为嗜水气单胞菌的蛋白。 - For protein identification , proteins spots of interest on the gels stained with colloidal coomassie brilliant blue g - 250 were excised , digested in - gel with trypsin , and analyzed by peptide mass fingerprinting ( pmf ) with matrix assisted laser desorption / ionization - mass spectrometry ( maldi - ms ) . erp60 , trypsinogen , proelastase , lipaseandso on were identified . this will enable us to present an overview of the proteins expressed in rat pancreas tissues and lays the basis for subsequent comparative proteome analysis studies with pancreas development
从中随机选择一些差异蛋白质点,进行基质辅助激光解吸-电离飞行时间质谱( maldi - tof - ms )测定其胶内酶解后的肽质指纹图谱,用mascot软件查询swiss - port数据库,初步鉴定为一些与生长发育、物质代谢、细胞因子、信号转导等有关的蛋白质,如erp60 、 trypsinogen 、 proelastase 、 lipase等。